Immunomedulatory Effects of Single and Combin Atorial Ant I- Cancer Chemotherapy in a Tumor Mouse Model

Anticancer chemotherapy is a successful treatment of tumor to k il l or to low the mult iplying rate of tumor cells. Although the immunomodulatory ef fect of single chemotherapy has been invest igated, the effects of combinatorial chemotherapy are i ll defined. Therefore the aim of this study was to evaluate effects of CTX and CIS alone or in combination as convent ional chemotherapeut ic drugs on the total and differential numbers of leukocytes. Groups of adult female Swiss albino (CD1) mice were treated with intraperitoneal ( i.p.) injection of 0.5 x 10 cells/mouse of Ehrlichs ascites carcinoma (EAC) cells. After one day of injection, mice were i.p. treated either with PBS, (10 μg/mouse) CIS, (4 mg/mouse) CTX or in combinat ion with different sequent ial treatment protocol, m ice were bled on day11 and complete blood count (CBC) was assessed. Treatment with low dose of (10 μg/mouse) CIS or with high dose of (4 mg/mouse) CTX followed by (10 μg/mouse) CIS induced slight decreases in the total numbers of white blood cells (WBCs) and absolute numbers of neutrophils and lymphocytes. Treatment with high dose (4 mg/mouse) of CTX or with low dose of (10 μg/mouse) CIS followed by CTX (4mg/mouse) induced marked decreases in the total numbers of WBCs, relative and absolute numbers of neutrophils and lymphocytes. The data suggested that certain sequent ial treatment protocol of high dose of chemotherapy can induce lower lymphopenia, which have important implications in cancer treatment.